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Synergistic antitumor effect of nanomedicine induced <t>ferroptosis</t> and ICD. (A) Morphological changes of cells treated with nanomedicine (bright-field imaging). (B) Cell viability evaluation by Cell Counting Kit-8 (CCK-8). (C) Lipid peroxidation level and SLC7A11 gene expression regulation. (D) Correlation analysis between GSH depletion and iron death markers. (E) Quantification of cell membrane damage (LDH release). (F) ATP concentration dynamics and immunogenic death activation. (G) HMGB1 release level and DAMP signal intensity. * P < 0.05; ** P < 0.01; *** P < 0.001.
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Synergistic antitumor effect of nanomedicine induced <t>ferroptosis</t> and ICD. (A) Morphological changes of cells treated with nanomedicine (bright-field imaging). (B) Cell viability evaluation by Cell Counting Kit-8 (CCK-8). (C) Lipid peroxidation level and SLC7A11 gene expression regulation. (D) Correlation analysis between GSH depletion and iron death markers. (E) Quantification of cell membrane damage (LDH release). (F) ATP concentration dynamics and immunogenic death activation. (G) HMGB1 release level and DAMP signal intensity. * P < 0.05; ** P < 0.01; *** P < 0.001.
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Synergistic antitumor effect of nanomedicine induced <t>ferroptosis</t> and ICD. (A) Morphological changes of cells treated with nanomedicine (bright-field imaging). (B) Cell viability evaluation by Cell Counting Kit-8 (CCK-8). (C) Lipid peroxidation level and SLC7A11 gene expression regulation. (D) Correlation analysis between GSH depletion and iron death markers. (E) Quantification of cell membrane damage (LDH release). (F) ATP concentration dynamics and immunogenic death activation. (G) HMGB1 release level and DAMP signal intensity. * P < 0.05; ** P < 0.01; *** P < 0.001.
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imidazole  (MedChemExpress)
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Synergistic antitumor effect of nanomedicine induced <t>ferroptosis</t> and ICD. (A) Morphological changes of cells treated with nanomedicine (bright-field imaging). (B) Cell viability evaluation by Cell Counting Kit-8 (CCK-8). (C) Lipid peroxidation level and SLC7A11 gene expression regulation. (D) Correlation analysis between GSH depletion and iron death markers. (E) Quantification of cell membrane damage (LDH release). (F) ATP concentration dynamics and immunogenic death activation. (G) HMGB1 release level and DAMP signal intensity. * P < 0.05; ** P < 0.01; *** P < 0.001.
Imidazole, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Synergistic antitumor effect of nanomedicine induced ferroptosis and ICD. (A) Morphological changes of cells treated with nanomedicine (bright-field imaging). (B) Cell viability evaluation by Cell Counting Kit-8 (CCK-8). (C) Lipid peroxidation level and SLC7A11 gene expression regulation. (D) Correlation analysis between GSH depletion and iron death markers. (E) Quantification of cell membrane damage (LDH release). (F) ATP concentration dynamics and immunogenic death activation. (G) HMGB1 release level and DAMP signal intensity. * P < 0.05; ** P < 0.01; *** P < 0.001.

Journal: Cyborg and Bionic Systems

Article Title: Nano-Immunotherapy Synergizing Ferroptosis and STING Activation in Metastatic Bladder Cancer

doi: 10.34133/cbsystems.0458

Figure Lengend Snippet: Synergistic antitumor effect of nanomedicine induced ferroptosis and ICD. (A) Morphological changes of cells treated with nanomedicine (bright-field imaging). (B) Cell viability evaluation by Cell Counting Kit-8 (CCK-8). (C) Lipid peroxidation level and SLC7A11 gene expression regulation. (D) Correlation analysis between GSH depletion and iron death markers. (E) Quantification of cell membrane damage (LDH release). (F) ATP concentration dynamics and immunogenic death activation. (G) HMGB1 release level and DAMP signal intensity. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The ferroptosis inducer IKE (imidazole ketone erastin), STING agonist diABZI, and anti-PD-1 antibody (aPD-1) were obtained from MedChemExpress.

Techniques: Imaging, Cell Counting, CCK-8 Assay, Gene Expression, Membrane, Concentration Assay, Activation Assay